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We sequenced the genome and transcriptome of 3 male and 3 individuals that are female each one of the 4 target types

Donnerstag, Februar 13th, 2020

We sequenced the genome and transcriptome of 3 male and 3 individuals that are female each one of the 4 target types

Outcomes and Discussion

(P. Wingei, P. Picta, Poecilia latipinna, and Gambusia holbrooki) (SI Appendix, Table S1) selected to express a also taxonomic circulation across Poeciliidae. For each species, we created DNA sequencing (DNA-seq) with on average 222 million 150-base set (bp) paired-end reads (average insert measurements of 500 bp, leading to on average 76-fold protection) and 77.8 million 150-bp mate-pair reads (average insert size of 2 kb, averaging 22-fold protection) per person. We additionally created, an average of, 26.6 million 75-bp paired-end RNA-seq checks out for each person.

Past focus on the sex chromosomes of the types revealed proof for male heterogametic systems in P. Wingei (48), P. Picta (50), and G. Holbrooki (51), and a lady system that is heterogametic P. Latipinna (52, 53). For every single target species, we built a de that is scaffold-level genome assembly using SOAPdenovo2 (54) (SI Appendix, Table S2). Each installation ended up being built utilizing the reads through the sex that is homogametic to be able to avoid coassembly of X and Y reads. (mehr …)